UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 2 > J.D. Evans1, D.L. Thornton2 and S.L. Branton1

Diagnosis of Mycoplasma gallisepticum from a Broiler Breeder Flock: Comparison of Three Diagnostics Methods

J.D. Evans1, D.L. Thornton2 and S.L. Branton1
1USDA-Agricultural Research Service, Poultry Research Unit, Mississippi State, MS 39762, USA 2Mississippi Board of Animal Health, Carthage, MS 39051, Mississippi State University, Mississippi State, MS 39762, USA
Abstract :

NPIP-mandated serological screening of a multiplier breeder flock detected possible Mycoplasma
gallisepticum (MG) exposure. The flock was quarantined and further samples including blood and choanal
swabs were collected and sent to a research facility for independent testing and confirmation. Subsequent
analyses included diagnosis by Serum Plate Agglutination (SPA), MG-specific Polymerase Chain Reaction
(PCR) and culture identification. Results of the various diagnostic tests were compared. The findings of the
various diagnostic tests were in agreement and confirmed MG infection of the breeder flock. Time
requirements of the various diagnostic procedures were recorded and were 1 h, 27 h and 30 days for SPA,
MG-specific PCR and culture identification, respectively. The results affirm the validity of the diagnostic
procedures and emphasize the importance of timely screening and diagnostic procedures for control of MG.

Keywords :
Mycoplasma gallisepticum, diagnostics, chronic respiratory disease, poultry, broiler breeders, NPIP

Date Deposited : 05 Jul 2011 10:32

Last Modified : 05 Jul 2011 10:32

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 2, - 2009 , ISSN 1682-8356

Download:
Full Text Original
Abstract : pdf doc