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.: Home > Nature and Science > 2004 > Volume 2 Number 3 > Qiang Zhang, Xuejun Gao, Shanshan Qian, Minyue Ma, Qingzhang Li, Yingjun Cui

Preparation of Porcine Factor V

Qiang Zhang, Xuejun Gao, Shanshan Qian, Minyue Ma, Qingzhang Li, Yingjun Cui
(Veterinary College, Northeast Agricultural University, Harbin, Heilongjiang 150030, China)
Abstract :

A rapid, simple, and reproducible method for the preparation of porcine Factor V from porcine
plasma was investigated in this study. Methods: Porcine  Factor  V was isolated by multi-techniques
including polyethylene glycol precipitation, absorption of protein on to barium citrate and DEAE-cellulose
chromatography. Results:  Porcine Factor V was identified by  SDS-PAGE. Yields and purity of  porcine
Factor  V  were 90.2 mg/L of  starting plasma and 64.4%, respectively.  Conclusion:  This preparative
procedure is simple, high yields and suitable for mass production. [Nature and Science. 2004;2(3):87-90].

Keywords :
porcine plasma; Factor V; preparation

Date Deposited : 11 Jan 2011 14:59

Last Modified : 12 Jan 2011 10:08

Official URL: http://www.sciencepub.org/nature/0101/

Volume 2, Number 3, October 2004 , ISSN 1545-0740

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