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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 2 > Arthur Hinton, Jr. and Ronald Holser

Role of Water Hardness in Rinsing Bacteria from the Skin of Processed Broiler Chickens

Arthur Hinton, Jr. and Ronald Holser
Russell Research Center, 950 College Station Road, Agricultural Research Service, United States Department of Agriculture, Athens, GA 30605, USA
Abstract :

The effect of water hardness on the ability of water to rinse bacteria from broiler skin was
examined. Very hard water (total hardness = 200 ppm) was prepared by dissolving calcium chloride and
magnesium chloride in distilled (soft) water and moderately hard water (total hardness = 100 ppm) was
prepared by diluting 1 part very hard with 1 part soft water. After five consecutive rinses of skin in soft,
moderately hard, or very hard water, samples were stomached in 0.01 M potassium phosphate buffer with
0.025% ethylenediaminetetraacetic acid to recover bacteria remaining on the skin. Bacteria in stomached
rinsates were enumerated on Plate Count (PC), Levine Eosine Methylene Blue (EMB), Campylobacter (CA),
Pseudomonas (PS) and Staphylococci (ST) Agars. Results indicated that significantly (p<0.05) fewer bacteria were recovered on CA and PS Agars from skin rinsed in soft water than from skin rinsed in moderately or very hard water, and fewer bacteria were recovered on EMB Agar from skin rinsed in soft water than from skin rinsed in very hard water. Skin was also rinsed in very hard water that had been softened by adding 0, 1.0, 2.5, or 5.0% potassium citrate. Results indicated that fewer bacteria were recovered on EMB and CA Agars from skin rinsed in water softened with 5.0% citrate than from skin rinsed in water with 0, 1.0, or 2.5% citrate. Chemically softened water was not bactericidal. Findings indicate that reducing water hardness may increase the ability of water to remove bacteria from broiler skin.

Keywords :
water hardness, water softeners, broiler skin, bacteria

Date Deposited : 05 Jul 2011 10:44

Last Modified : 05 Jul 2011 10:44

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 2, - 2009 , ISSN 1682-8356

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