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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 3 > B.P. Shankar1, R.N.S. Gowda1, B.H. Manjunath Prabhu1, B. Pattnaik2, S. Nagarajan2, S.S. Patil2 and H.K. Pradhan2

Assessment of Pathogenic Potential of Two Indian H5N1 Highly Pathogenic Avian Influenza Virus Isolates by Intravenous Pathogenicity Index Test

B.P. Shankar1, R.N.S. Gowda1, B.H. Manjunath Prabhu1, B. Pattnaik2, S. Nagarajan2, S.S. Patil2 and H.K. Pradhan2
1Department of Pathology, Veterinary College, Hebbal, Bangalore - 24, Karnataka, India 2High Security Animal Disease Laboratory, IVRI, Bhopal, India
Abstract :

Intravenous Pathogenicity Index (IVPI) test was conducted to assess the pathogenicity of two H5N1
(A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06) AIV isolates. Both the H5N1 virus isolates were isolated from
natural outbreaks during 2006, both isolates caused death of all birds with in 48 h after inoculation
experimentally, birds showed typical clinical signs, gross and microscopic lesions of Avian influenza. IVPI
of A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06 was 2.96 and 2.95 respectively. This test showed that both H5N1
isolates are highly pathogenic. Virus could be re-isolated from all the organs of infected chickens and it was reconfirmed by RT-PCR using WHO and Lee primers.

Keywords :
HPAI, ivpi, india, rt-pcr and pathology

Date Deposited : 05 Jul 2011 10:51

Last Modified : 05 Jul 2011 10:51

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 3, - 2009 , ISSN 1682-8356

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