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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 5 > B.P. Shankar1, R.N. Sreenivas Gowda2, B. Pattnaik1, B.H. Manjunatha Prabhu3, R.P. Kamal1, B.K. Sreenivas4, H.S. Madhusudhan2, D. Ranjith2 and H.K. Pradhan1

Assessment of Pathogenic Potential of Avian Influenza Viruses by MDCK Cell Culture

B.P. Shankar1, R.N. Sreenivas Gowda2, B. Pattnaik1, B.H. Manjunatha Prabhu3, R.P. Kamal1, B.K. Sreenivas4, H.S. Madhusudhan2, D. Ranjith2 and H.K. Pradhan1
1High Security Animal Disease Laboratory, IVRI, Bhopal, India 2Veterinary College, Hebbal, Bangalore-24, Karnataka, India 3Molecular Biology Genetics Units, JNCASR, Jakkur, Bangalore, India 4IAH&VB, Hebbal, Bangalore, India
Abstract :

The influenza viruses are sub-classified in to two pathotypes of Highly Pathogenic Avian Influenza
(HPAI) and Low Pathogenic Avian Influenza (LPAI) viruses on the basis of the pathogenicity of AIV in domestic poultry. Samples of H5N1 (7966/06 and 7972/06) and the H9N1 (5844/05) viruses were also grown on MDCK cell. All these produced cytopathic effect within 72 h. Normally, the nonpathogenic AIV does not produce CPE in MDCK cells. However, CPE can be produced if trypsin is incorporated while, culturing the viruses. In the present study, without the addition of trypsin the viruses produced CPE. On FAT both cytoplasmic and nuclear florescence was observed. It is also known that the viruses which produce CPE in absence of trypsin are pathogenic. It proved beyond that the H5N1 and H9N1 viruses isolated in the present study were pathogenic based on cell culture study.

Keywords :
Avian influenza H5N1, MDCK, CPE, India

Date Deposited : 05 Jul 2011 12:51

Last Modified : 05 Jul 2011 12:51

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 5, - 2009 , ISSN 1682-8356

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