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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 5 > Josie Maria Campioni1,2, Antonio Carlos Paulillo3,4, Elizabeth Moreira dos Santos Schmidt3,5, Marcia Nishizawa6, Alan Jonathan Pereira Testi7, Janine Denadai1 and Adriano de Oliveira Torres Carrasco3

Evaluation of Experimental Vaccination in Chinese Goose (Anser cygnoides) Against Newcastle Disease: Investigation of the State of Virus Carrier

Josie Maria Campioni1,2, Antonio Carlos Paulillo3,4, Elizabeth Moreira dos Santos Schmidt3,5, Marcia Nishizawa6, Alan Jonathan Pereira Testi7, Janine Denadai1 and Adriano de Oliveira Torres Carrasco3
1Aluna Programa de Pَs-graduacao em Medicina Veterinaria da FCAV-Unesp, Jaboticabal, Brazil 2Bolsista CAPES, Brazil 3Departamento de Patologia Veterinaria, FCAV, Unesp, Jaboticabal, Brazil 4Research Fellow CNPq-Brazil 5Post-Doctorate Researcher in Veterinary Medicine, FAPESP, Brazil 6Docente, Universidade Metodista de Sao Paulo, Sao Bernardo do Campo, Brazil 7Aluno Curso Graduacao Medicina Veterinaria, FCAV, Unesp, Jaboticabal, Bolsista PIBIC/CNPq, Brazil
Abstract :

This study aimed the characterization of the importance of vaccination against Newcastle disease
in Chinese geese (Anser cygnoides) and to investigate the state of carrier of the virus in this species. There were used 120 Chinese geese, distributed at random into 4 groups, vaccinated or not. At 60 days of age, all groups were challenged with a pathogenic virus (NDV) suspension, EID50 = 10 /0.1 mL and a group of 8.15 Specific Pathogen Free (SPF) chickens were used as control of the virus. Cloacal and tracheal swabs were collected after 6, 10 and 20 days post-challenge for genome viral excretion by RT-PCR (reverse transcriptionpolymerase chain reaction). Chinese goose of all groups did not demonstrate any signs of Newcastle disease. They were refractory to the clinical disease with the NDV. In Chinese geese from control group, NDV genome was detected 20 days after challenge. It was demonstrated therefore the state of carrier of NDV by Chinese goose. In geese, from the vaccinated groups, genome viral excretion was not detected by RT-PCR. It was also demonstrated the importance of the vaccination in the suppression of the state of carrier of NDV in Chinese geese.

Keywords :
Newcastle disease, chinese geese, Anser cygnoides, NDV carrier, vaccination

Date Deposited : 05 Jul 2011 13:06

Last Modified : 05 Jul 2011 13:06

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 5, - 2009 , ISSN 1682-8356

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