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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 6 > Adriano Sakai Okamoto, Raphael Lucio Andreatti Filho, Ticiana Silva Rocha,Anita Menconi and Guilherme Augusto Marietto-Gonçalves

Relation Between the SpvC and InvA Virulence Genes and Resistance of Salmonella enterica Serotype Enteritidis Isolated from Avian Material

Adriano Sakai Okamoto, Raphael Lucio Andreatti Filho, Ticiana Silva Rocha,Anita Menconi and Guilherme Augusto Marietto-Gonçalves
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
Abstract :

Pathogenic bacteria possess genes responsible for virulence, that when expressed, determine
not only bacterial invasion and persistence and destruction of host cells but also survival capacity under
inappropriate culture conditions. This study utilized 100 samples of Salmonella enterica subspecies enterica Serotype Enteritidis (SE) isolated from avian material, detected the virulence genes InvA and SpvC by medium of Polymerase Chain Reaction (PCR) and compared them for possible expression of virulence
factors for survival in hostile conditions of temperature, pH and reduced concentration of nutrients  necessary for SE multiplication. Of all the samples analyzed, two presented the genes SpvC and InvA, simultaneously, with one probable expression of them being verified in growth with pH 10.0 or temperature of 25oC. But in relation to nutrient concentration, neither sample obtained growth when seeded in medium containing 0.5% nutrient.

Keywords :
Gene InvA, gene SpvC, resistance, Salmonella enteritidis, virulence

Date Deposited : 06 Jul 2011 08:33

Last Modified : 06 Jul 2011 08:33

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 6, - 2009 , ISSN 1682-8356

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