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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 7 > G. Dhinakar Raj, T.M. Chozhavel Rajanathan, K. Kumanan and S. Elankumaran

Expression Profile of Toll-Like Receptor mRNA in an Indigenous Aseel Breed of Chicken in India

G. Dhinakar Raj, T.M. Chozhavel Rajanathan, K. Kumanan and S. Elankumaran
Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
Abstract :

Expression profile of chicken six Toll-like Receptor (TLR) mRNAs were analyzed in the heterophils,
lungs, liver, spleen, duodenum, caecal tonsils and kidneys of 12-weeks old indigenous village chicken in
India, using reverse transcription polymerase chain reaction (RT-PCR). TLR 2 type 1 mRNA was expressed in lungs, liver, spleen, duodenum and caecal tonsils. TLR2 type 2 mRNA was expressed only in the lungs. TLR 3 mRNA was expressed in lungs, liver, spleen and caecal tonsils. TLR 4 mRNA was expressed only in lungs, liver and spleen. TLR 5 and TLR 7 mRNAs were expressed in all the tissues examined. With respect to tissues, heterophils and lungs expressed all the TLR mRNAs examined while kidneys expressed only TLR 5 and TLR 7 mRNAs. All the TLR amplified PCR products were partially sequenced and showed high homologies with the available chicken (commercial) TLR gene sequences. There could be a possibility of correlation between TLR mRNA expressions with higher levels of innate immunity seen in indigenous village breeds of chickens.

Keywords :
Toll-like receptors, expression profiles, RT-PCR, indigenous chicken

Date Deposited : 06 Jul 2011 09:07

Last Modified : 06 Jul 2011 09:07

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 7, - 2009 , ISSN 1682-8356

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