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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 8 > Essam S. Soliman1, Mohamed A.A. Sobeih2, Z.H. Ahmad2, M.M. Hussein2 and H.A.A. Moneim3

Evaluation of Commercial Disinfectants Against Bacterial Pathogens Isolated from Broiler Farms

Essam S. Soliman1, Mohamed A.A. Sobeih2, Z.H. Ahmad2, M.M. Hussein2 and H.A.A. Moneim3
1Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama 36088, USA 2Department of Animal Hygiene, Zoonoses and Animal Behavior, College of Veterinary Medicine, Suez Canal University, Ismailia, Egypt 3Department of Clinical Pathology, College of Medicine, Suez Canal University, Ismailia, Egypt
Abstract :

Five disinfectants [TH4® (combination of quaternary ammonium compounds and gluteraldhyde),
Microzal® (combination of quaternary ammonium compounds and gluteraldhyde), Incospect IC 22XA
(combination of quaternary ammonium compounds, gluteraldhyde and formalin), Povidone Iodine®
(iodophore) and Formalin® (commercial formaldehyde 37%) were tested in a laboratory trials against four bacterial isolates (Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas
aureuginosa) at concentration of (~105) isolated during epidemiological surveillance. The trials were carried in the presence and absence of organic matter (dried yeast 5%) using MIC use-dilution test. Minutely samples were collected for the bacterial counts. In the absence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 5, 5, 10 and 5 min (p<0.0001) respectively, Microzal® and Incospect IC 22XA achieved the 100% killing efficacy against the four Micro-organisms after 5 min (p<0.0001), Povidone Iodine® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively and Formalin® achieved the 100% killing efficacy after 5, 5, 5 and 20 min (p<0.0001) respectively. In the presence of organic matter, TH4® achieved the 100% killing efficacy against Staphylococcus aureus, Escherchia coli, Klebsiella oxytoca and Pseudomonas aureuginosa after 10, 5, 20 and 20 min (p<0.0001) respectively, Microzal® achieved the 100% killing efficacy after 5, 5, 5 and 10 min (p<0.0001) respectively, Incospect IC 22XA achieved the 100% killing efficacy after 5, 5, 10 and 5 min (p<0.0001) respectively, Povidone Iodine® achieved the 100% killing efficacy against the four micro-organisms after 30 min (p<0.0001) and Formalin® achieved the 100% killing efficacy after 30, 5, 20 and 20 min (p<0.0001) respectively. The results revealed that quaternary ammoniumgluteraldhyde  combination (TH4®, Microzal® and Incospect IC 22XA) although they are not proven to be  environmentally safe; they are the most powerful disinfectants because of the synergistic action of the
quaternary ammonium and gluteraldhyde bases.

Keywords :
Disinfectants, evaluation, bacterial pathogens, broiler

Date Deposited : 06 Jul 2011 10:02

Last Modified : 06 Jul 2011 10:02

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 8, - 2009 , ISSN 1682-8356

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