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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2009 > Volume 8 Number 9 > Adriana Kolesarova1, M. Capcarova1, A. Sirotkin2 and P. Massanyi1

Insulin-Like Growth Factor-I and Progesterone Release by Ovarian Granulosa Cells of Hens after Experimental Lead and Molybdenum Administrations in vitro

Adriana Kolesarova1, M. Capcarova1, A. Sirotkin2 and P. Massanyi1
1Department of Animal Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, SK-949 76, Slovak Republic 2Department of Genetics and Reproduction, Animal Production Research Centre, Nitra, 95141, Luzianky, Slovak Republic
Abstract :

The general objective of this in vitro study was to examine the secretory activity of ovarian granulosa
cells of hens after lead (Pb) and molybdenum (Mo) administrations. In this study the effect of various doses
of Pb and Mo on the insulin-like growth factor-I (IGF-I) and progesterone (P ) release by ovarian granulosa 4 cells was demonstrated. Ovarian granulosa cells were incubated with lead acetate/ammonium molybdategroup A 0.09 mg/mL, group B 0.17 mg/mL, group C 0.33 mg/mL and the control group without Pb and Mo administrations for 18 h. The IGF-I and progesterone release by granulosa cells of hens was assessed by RIA. It was demonstrated that isolated ovarian cells were able to survive in culture and release hormones IGF-I and P after experimental Pb and Mo administrations. Our observations demonstrated that the secretory 4 activities of granulosa cells of hens were dependent on Pb and Mo doses. Lower Pb concentrations (0.09 mg/mL and 0.17 mg/mL) induced IGF-I release but higher dose (0.33 mg/mL) stimulated P release by 4 ovarian granulosa cells of hens. Higher Mo concentration (0.33 mg/mL) reduced of IGF-I release while doses 0.17 mg/mL and 0.33 mg/mL stimulated P release by ovarian cells of hens. These findings contribute to our 4 knowledge of the effect of lead and molybdenum on ovarian function of hens.

Keywords :
Lead, molybdenum, granulosa cells, ovary, hens

Date Deposited : 06 Jul 2011 11:15

Last Modified : 06 Jul 2011 11:15

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 8, Number 9, - 2009 , ISSN 1682-8356

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