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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2008 > Volume 7 Number 2 > Gary Garcia - Espinosa1, Stefan Clerens2, Lut Arckens2, Gisela F. Erf3, G. Tellez3 and Billy M. Hargis3

Peptides from the Bursa of Fabricius Associated with Suppression of Mitogen Stimulated DNA - Synthesis in Bursa of Fabricius Cells Belong to Intracellular Proteins

Gary Garcia - Espinosa1, Stefan Clerens2, Lut Arckens2, Gisela F. Erf3, G. Tellez3 and Billy M. Hargis3
1Departamento de Producciَn Animal, Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autَnoma de México, 04510, México, D.F. 2Laboratory of Neuroplasticity and Neuroproteomics Katholieke Universiteit Leuven, Leuven B-3000, Belgium 3Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA
Abstract :

Previous reports from our laboratory have demonstrated that an reverse phase HPLC (rpHPLC) fraction obtained from extracts of the chicken bursa of Fabricius posses both in vitro anti-steroidogenic activity on avian and mammalian cells and suppression on mitogen stimulated DNA-synthesis in chicken BF cells. Utilizing YM cut-off membranes the bioactive fraction appears to be between ~3-5kDa. However, the identity of such peptide (s) remains unknown. Here, subjected those peptides for mass spectrometric (MS) nano-electrospray quadruple time-of-flight (Q-TOF) MS/MS analysis in an effort to elucidate the composition
of predominant fragments. The results of these analyses indicate the presence of small fragments of the
non-histone chromosomal protein high mobility group (HMG), nucleophosmin, elongation factor 1-alpha,
thymosin $4 (T$4), thymosin $, stathmin and histone H1.10. These results indicate that the suppression
present in the rpHPLC fraction obtained from the BF, rather than been recognized extracellular messengers, like hormones or cytokines, contains intracellular molecules.

Keywords :
Intracellular, anti-proliferative, anti-steroidogenic and bursa of Fabricius

Date Deposited : 07 Jul 2011 13:48

Last Modified : 07 Jul 2011 13:48

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Volume 7, Number 2, - 2008 , ISSN 1682-8356

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