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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2008 > Volume 7 Number 6 > Sunday Blessing Oladele1, King Akpofure Nelson Esievo1, Paul Abdu2 and Andrew Jonathan Nok3

In vitro Effects of Calcium, Magnesium, Mercury and Copper Ions on Neuraminidase of a Nigerian Newcastle Disease Virus Strains

Sunday Blessing Oladele1, King Akpofure Nelson Esievo1, Paul Abdu2 and Andrew Jonathan Nok3
1Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria 2Department of Surgery and Medicine, Ahmadu Bello University, Zaria, Nigeria 3Department of Biochemistry, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
Abstract :

This study was carried out to determine the effects of cations on neuraminidase during NDV infections in poultry. A total of two hundred samples from dialyzed neuraminidase of NDV Kudu 113 strain were used for the experiment. Fifty samples each were used on four divalent cations, namely calcium (Ca2+), magnesium (Mg2+), mercury (Hg2+) and copper (Cu2+) ions. Neuraminidase activity of NDV Kudu 113 strain was determined in vitro in the presence of these cations, tested as their chloride form by periodate
thiobarbituric acid assay method. Neuraminidase activity was also determined in the control samples that
were without cations. Out of the four divalent cations examined, Hg2+ induced the highest neuraminidase
activity of 75% at the concentration of 0.24M. Magnesium and Ca2+ ions also increased the activity of
neuraminidase of NDV Kudu 113 strain in vitro. On the other hand, Cu2+ significantly inhibited the activity of
this enzyme by 69.23% at the concentration of 0.47M. It was concluded that Ca2+, Mg2+ and Hg2+ induced
increase in the activity of neuraminidase of NDV Kudu 113 strain, while Cu2+ inhibited its activity in vitro.
Further studies should be carried out on the roles of these cations in Newcastle disease (ND) and the
possible use of Cu2+ in the future as inhibitor of the activity of neuraminidase of NDV in vivo, so as to reduce the menance of ND in poultry.

Keywords :
Cation, effect, neuraminidase and newcastle disease virus

Date Deposited : 12 Jul 2011 11:29

Last Modified : 12 Jul 2011 11:29

Official URL: http://www.pjbs.org/ijps/ijps.htm

Volume 7, Number 6, - 2008 , ISSN 1682-8356

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