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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2008 > Volume 7 Number 6 > H.M. Elsheikha1, A.T. Abbas2, M.M. Abdel Aziz2 and L.M. Elshabiny3

Therapeutic Efficacy of Chicken Egg Yolk Immunoglobulins Against Mycoplasma gallisepticum Infection in Chickens

H.M. Elsheikha1, A.T. Abbas2, M.M. Abdel Aziz2 and L.M. Elshabiny3
1Division of Veterinary Medicine, School of Veterinary Medicine and Science, University of Nottingham, College Road, Sutton Bonington Campus, Leicestershire, LE12 5RD, United Kingdom 2Department of Biotechnology, Gastroenterology Surgery Center, Mansoura University, Mansoura 35516, Egypt 3Animal Health Research Institute, Agriculture Research Center, Dokki, Giza, Egypt
Abstract :

The focus of this report was to assess the role of passive immunization using immunoglobulins derived from chicken egg yolk in protection against M. gallisepticum infection in chickens. Immunoglobulins
were produced by egg yolk technology. ELISA was used to evaluate their immunoreactivity and western
immunoblotting demonstrated that chicken egg yolk immunoglobulins Y (IgY) were capable of recognizing
M. gallisepticum immunoreactive antigens. Administration of IgY to experimentally infected broiler chickens
afforded up to 70% protection rate as confirmed by culture analyses. Challenge of IgY - treated birds with
M. gallisepticum virulent strain S6 revealed significantly (P< 0.05) lower isolation prevalence in comparison
to control chickens treated with IgY from mock-immunized (control) hens that received the same bacterial
challenge. Additionally, M. gallisepticum culture analysis from respiratory organs demonstrated that these
antibodies protected chickens in a dose-dependent pattern against M. gallisepticum infection (i.e., increasing IgY titer resulted in decreased isolation rate). These findings demonstrate that IgY obtained from hens immunized by M. gallisepticum could provide an alternative approach for treatment of M. gallisepticum infection in chickens.

Keywords :
Mycoplasma gallisepticum, egg-yolk antibodies, immunoglobulin therapy, passive antibody transfer

Date Deposited : 12 Jul 2011 11:41

Last Modified : 12 Jul 2011 11:41

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Volume 7, Number 6, - 2008 , ISSN 1682-8356

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