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.: Home > International Journal of Medical Sciences > 2010 > Volume 7 Number 3 > Takashi Kuwahara1.., Kazuyuki Shimono1, Shinya Kaneda1, Takumi Tamura1, Masao Ichihara2, Yoshifumi Nakashima1

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Takashi Kuwahara1.., Kazuyuki Shimono1, Shinya Kaneda1, Takumi Tamura1, Masao Ichihara2, Yoshifumi Nakashima1
1. Preclinical Assessment Department, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan. 2. Research and Development Center, Otsuka Pharmaceutical Factory,
Abstract :

Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions containing lipid. Methods: The pH value of a TPN solution containing lipid (pH 6.0, containing 20 ppm of NaHSO3) was adjusted by the addition of HCl to 5.7, 5.4, or 4.9. The pH value of another TPN solution (pH5.5, containing 400 ppm of NaHSO3) was adjusted by the addition of NaOH to 5.9, 6.3, or 6.8. A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0, 24, and 48 hrs later. Results: C albicans increased similarly at any pH values in the TPN solution. The bacterial  species also increased rapidly at pH6.0 in the solution containing 20 ppm of NaHSO3, but growth was suppressed as the pH value was reduced, with growth halted at pH4.9. However, these bacterial species did not increase, even at pH5.9, in the other solution containing 400 ppm of NaHSO3. Conclusions: These results suggest that Candida species can grow rapidly in almost all TPN solutions regardless of the acidity, lipid, and NaHSO3; also, some bacterial species may grow in TPN solutions containing lipid unless the pH value is 5.0 or less. Therefore, each TPN solution should be examined whether or not the bacterial species can proliferate.

Keywords :
CRBSI, microbial growth, TPN solution, lipid, pH, bisulfite

Date Deposited : 27 Jul 2011 12:33

Last Modified : 27 Jul 2011 12:33

Official URL: http://www.medsci.org/archive

Volume 7, Number 3, - 2010 , ISSN 1449-1907

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