UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2009 > Volume 6 Number 1 > Ana Lustig1, Arnell Carter1, Dorothy Bertak1, Divya Enika1, Bolormaa Vandanmagsar1, William Wood2, Kevin G. Becker2, Ashani T. Weeraratna1 and Dennis D. Taub1

Transcriptome analysis of murine thymocytes reveals age-associated changes in thymic gene expression

Ana Lustig1, Arnell Carter1, Dorothy Bertak1, Divya Enika1, Bolormaa Vandanmagsar1, William Wood2, Kevin G. Becker2, Ashani T. Weeraratna1 and Dennis D. Taub1
1. Laboratory of Immunology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD. 21224, USA 2. The Research Resources Branch, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, MD. 21224, USA
Abstract :

The decline in adaptive immunity, naïve T-cell output and a contraction in the peripheral T cell receptor (TCR) repertoire with age are largely attributable to thymic involution and the loss of critical cytokines and hormones within the thymic microenvironment. To assess the molecular changes associated with this loss of thymic function, we used cDNA microarray analyses to examine the transcriptomes of thymocytes from mice of various ages ranging from very young (1 month) to very old (24 months). Genes associated with various biological and molecular processes including oxidative phosphorylation, T- and B- cell receptor signaling and antigen presentation were observed to significantly change with thymocyte age.
These include several immunoglobulin chains, chemokine and ribosomal proteins, annexin A2, vav 1 and several S100 signaling proteins. The increased expression of immunoglobulin genes in aged thymocytes could be attributed to the thymic B cells which were found to be actively producing IgG and IgM antibodies. Upon further examination, we found that purified thymic T cells derived from aged but not young thymi also exhibited IgM on their cell surface suggesting the possible presence of auto-antibodies on the surface thymocytes with advancing age. These studies provide valuable insight into the cellular and molecular mechanisms associated with thymic aging.

Keywords :
thymus, involution, aging, microarray, AGEMAP, thymocytes, caloric restriction

Date Deposited : 27 Jul 2011 12:50

Last Modified : 27 Jul 2011 12:50

Official URL: http://www.medsci.org/archive

Volume 6, Number 1, - 2009 , ISSN 1449-1907

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal