UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2009 > Volume 6 Number 2 > Amanda D. French1, Jennifer L. Fiori1 #, Tura C. Camilli1, Poloko D. Leotlela1, Michael P. O’Connell1, Brittany P. Frank2, Sarah Subaran2, Fred. E. Indig2, Dennis D. Taub1 and Ashani T. Weeraratna1

PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells

Amanda D. French1, Jennifer L. Fiori1 #, Tura C. Camilli1, Poloko D. Leotlela1, Michael P. O’Connell1, Brittany P. Frank2, Sarah Subaran2, Fred. E. Indig2, Dennis D. Taub1 and Ashani T. Weeraratna1
1. Laboratory of Immunology, National Institute on Aging, Baltimore, MD 21124, USA 2. Research Resources Branch, National Institute on Aging, Baltimore, MD 21124, USA
Abstract :

Cytoplasmic expression of claudin-1 in metastatic melanoma cells correlates to increased migration, and increased secretion of MMP-2 in a PKC dependent manner, whereas claudin-1 nuclear expression is found in benign nevi. Melanoma cells were transfected with a vector expressing CLDN-1 fused to a nuclear localization signal (NLS). Despite significant nuclear localization of claudin-1, there was still transport of claudin-1 to the cytoplasm. Phorbol ester treatment of cells transfected with NLS-claudin-1 resulted in an exclusion of claudin-1 from the nucleus, despite the NLS. To ascertain whether PKC or PKA were involved
in this translocation, we mutated the putative phosphorylation sites within the protein. We found that mutating the PKC phosphorylation sites to mimic a non-phosphorylated state did not cause a shift of claudin-1 to the nucleus of the cells, but mutating the PKA sites did. Mutations of either site to mimic constitutive phosphorylation resulted in cytoplasmic claudin-1 expression. Stable claudin-1 transfectants containing non-phosphorylatable PKA sites exhibited decreased motility. These data imply that subcellular localization of claudin-1 can be controlled by phosphorylation, dicating effects on metastatic capacity.

Keywords :
Claudin, melanoma, metastasis, PKC, PKA

Date Deposited : 27 Jul 2011 13:07

Last Modified : 27 Jul 2011 13:07

Official URL: http://www.medsci.org/archive

Volume 6, Number 2, - 2009 , ISSN 1449-1907

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal