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International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2008 > Volume 5 Number 6 > Chongsuk Ryouand Charles E. Mays

Prion propagation in vitro: are we there yet?

Chongsuk Ryouand Charles E. Mays
Sanders Brown Center on Aging and Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY, U.S.A.
Abstract :

Prion diseases are caused by proteinaceous pathogens termed prions. Although the details of the  mechanism of prion propagation are not fully understood, conformational conversion of cellular prion protein (PrPC) to misfolded, disease-associated scrapie prion protein (PrPSc) is considered the essential biochemical event for prion replication. Currently, studying prion replication in vitro is difficult due to the lack of a system which fully recapitulates the in vivo phenomenon. Over the last 15 years, a number of in vitro systems supporting PrPC conversion, PrPSc amplification, or amyloid fibril formation have been established. In this review, we describe the evolving methodology of in vitro prion propagation assays and discuss their ability in reflecting prion propagation in vivo.

Keywords :
prion disease, prion, cellular prion protein, disease-associated scrapie prion protein, in vitro conversion, in vitro prion amplification, prion infectivity

Date Deposited : 28 Jul 2011 12:44

Last Modified : 28 Jul 2011 12:44

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Volume 5, Number 6, - 2008 , ISSN 1449-1907

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