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The Journal of Poultry Science

.: Home > The Journal of Poultry Science > 2010 > Volume 47 Number 1 > Supamit Mekchay 1) 4), Tawatchai Teltathum 2) 4), Sutkhet Nakasathien 3), and Petai Pongpaichan 1)

Proteomic Analysis of Tenderness Trait in Thai Native and Commercial Broiler Chicken Muscles

Supamit Mekchay 1) 4), Tawatchai Teltathum 2) 4), Sutkhet Nakasathien 3), and Petai Pongpaichan 1)
1) Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Thailand, 2) Human and Animal Biotechnology, Graduate School, Chiang Mai University, Thailand, 3) Department of Agronomy, Faculty of Agriculture, Kasetsart University, Thailand, 4) Center for Agricultural Biotechnology: AG-BIO/PERDO-CHE, Thailand
Abstract :

The objective of this study was to identify protein markers for tenderness trait of Thai native and commercial broiler chicken muscles. The proteome of chicken muscle with highand low-shear force values was analyzed by two-dimensional gel electrophoresis and MALDI-TOF/MS technique. A total of 169 and 158 protein spots were observed in Thai native and commercial broiler chicken muscles, respectively. Of these proteins, five protein spots were upand down-regulated with low shear force values of chicken meat. Selected three protein spots were identified and showed homology with pyruvate kinase 2 muscle (PKM2), phosphoglycerate mutase 1 (PGAM1) and triosephosphate isomerase 1 (TPI1) of chicken. The PKM2 and TPI1 were correlated with shear force values of chicken meats. Whereas, the PGAM1, B46 and B107 trended toward an association with shear force values. The results indicate that these enzymes of the glycolytic pathway play a major role in the energy metabolism process of muscle and meat characteristics. These findings promote the importance of the muscle metabolic enzymes and could be used as functional candidate genes for meat quality traits in chicken.

Keywords :
broiler, gene expression, muscle, proteome, Thai native chicken

Date Deposited : 12 Jan 2011 14:53

Last Modified : 12 Jan 2011 14:53

Official URL: http://www.jstage.jst.go.jp/article/jpsa/47/1/47_8/_article

Volume 47, Number 1, - 2010 , ISSN 1349-0486 (online), ISSN 1346-7395 (print)

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