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International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2007 > Volume 4 Number 4 > Sّren Mّller1, Finn Laigaard1, Klaus Olgaard2, Claus Hemmingsen 1

Effect of 1,25-dihydroxy-vitamin D3 in experimental sepsis

Sّren Mّller1, Finn Laigaard1, Klaus Olgaard2, Claus Hemmingsen 1
1. Department of Anaesthesiology, Frederiksberg Hospital, University of Copenhagen, Denmark 2. Department of Nephrology, Rigshospitalet, University of Copenhagen, Denmark
Abstract :

Background: In addition to the regulation of calcium homeostasis, vitamin D affects the cellular immune system, targets the TNF-ل pathway and increases vasoconstrictor response to angiotensin II. We therefore examined the effect of 1,25-dihydroxy-vitamin D3 on coagulation and organ failure in experimental sepsis in the rat. Methods: Three series of placebo-controlled studies were conducted. All rats were pre-treated with daily SC injections of 1,25-dihydroxy-vitamin D3 100 ng/kg or placebo vehicle for 3 days. In study 1, sepsis was accomplished
by abdominal surgery comprising a coecal ligation and puncture with a 1,2 mm needle, or sham surgery. In study 2, the rats had a single IP injection of lipopolysaccharide from E. Coli 0111:B4 (LPS) 8 mg/kg, or placebo. In study 3, an hour-long IV infusion of LPS 7 mg/kg, or placebo was given. Results: All three models of sepsis showed significant effects on coagulation and liver function with reduced thrombocyte count and prothrombin time together with elevated ALT and bilirubin (p<0.05) as compared to controls. In study 1, the vitamin D treated rats maintained normal platelet count, whereas the vehicle treated rats showed a significant reduction (p<0.05). This effect of vitamin D on platelets was not found in the LPS-treated
groups. We found no significant differences between vitamin D and placebo-treated rats with regards to liver function. Conclusion: The present data suggest a positive modulating effect of 1,25-dihydroxy-vitamin D3 supplementation on sepsis-induced coagulation disturbances in the coecal ligation and puncture model. No such effect was found in LPS-induced sepsis.

Keywords :
1,25 Vitamin D, calcitriol, sepsis, rats, coagulation, thrombocytes

Date Deposited : 29 Jul 2011 10:04

Last Modified : 29 Jul 2011 10:04

Official URL: http://www.medsci.org/archive

Volume 4, Number 4, - 2007 , ISSN 1449-1907

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