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International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2006 > Volume 3 Number 1 > B S Jesuيno, C Casimiro, V E do Rosلrio and H Silveira

Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene

B S Jesuيno, C Casimiro, V E do Rosلrio and H Silveira
Centro de Malلria e Outras Doenças Tropicais, UEI Malلria, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 96, 1349-008 Lisbon, Portugal
Abstract :

Antibodies are known to play an important role in the control of malaria infection. However, they can modulate parasite development enhancing infection. The effect of anti-Plasmodium antibodies on the expression of circumsporozoite protein gene (csp) was investigated. Plasmodium falciparum 3D7 in vitro cultures were submitted to: i) anti- circumsporozoite protein monoclonal antibody (anti-CSP-mAb) [1ىg/ml, 0.1ىg/ml, 0.01ىg/ml and 0.001ىg/ml] and ii) purified IgG Fab fragment from a pool of malaria patients [1mg/ml and 1ىg/ml]; and compared to control cultures. After 24h the number of ring infected erythrocytes was determined in order to calculate invasion efficacy. At 48h culture supernatant was collected, and the amount of circumsporozoite protein determined by ELISA, parasitaemia was calculated and cells were processed for RNA preparation. Expression of csp gene was quantified using Real time RT-PCR. There was an increase in parasite growth when treated with lower anti-CSP-mAb concentration, which was associated with lower csp expression, while 1ىg/ml anti-CSP-mAb treatment presented a growth inhibitory effect accompanied by high csp expression.

Keywords :
Plasmodium falciparum, circumsporozoite protein, erythrocyte invasion

Date Deposited : 29 Jul 2011 12:35

Last Modified : 29 Jul 2011 12:35

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Volume 3, Number 1, - 2006 , ISSN 1449-1907

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