UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Medical Sciences

.: Home > International Journal of Medical Sciences > 2005 > Volume 2 Number 4 > Ashok Kumar 1 2, Pallab Ray 2, Mamta Kanwar 1 2, Meera Sharma 2, Subhash Varma 3

A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections

Ashok Kumar 1 2, Pallab Ray 2, Mamta Kanwar 1 2, Meera Sharma 2, Subhash Varma 3
1. Kresge Eye Institute, School of Medicine, Wayne State University, Detroit, MI 48201 USA 2. Departments of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India 160012 3. Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India 160012
Abstract :

Immunoblot and an enzyme-linked immunosorbent assays were used to evaluate and compare IgG antibodies against S. aureus whole cell lysate, cell wall peptidoglycan and lipoteichoic acid to discriminate between deep-seated and superficial S. aureus infection. Serum samples were examined from patients with deep-seated (n = 25) and superficial (n = 25) S. aureus infections and 15 healthy controls. Patients with deep-seated infections exhibited a large  number of immuno-reactive bands in their IgG immunoblot profile as compared to those with superficial infections and healthy controls. Anti-staphylococcal IgG antibodies that reacted with two antigens of apparent molecular weight 110 and 98 kDa were specifically present in 96% (24/25) of patients with deep-seated infections, and were absent in, superficial and control sera. Moreover other Gram-positive and Gram-negative bacteria did not share these two unique antigens. The ELISA assays revealed significantly elevated levels of IgG antibodies to peptidoglycan (PG) in 18 of 25 (72%) patients with deep infection and 15 of 25 (60%) patients with superficial staphylococcal infection. The elevated levels of IgG antibodies to teichoic acid (TA) antigen were detected in all (100%) deep-seated group patients and among 40% (10/25)
patients with superficial infection. An increase in levels of antibodies to PG showed a positive correlation trend with levels of IgG antibodies to TA only in deep infection group. Thus immunoblot detection of these two unique antigens as well as detection of elevated antibodies against PG and TA may be useful for the discrimination of staphylococcal deepseated and superficial infection in humans.

Keywords :
S. aureus, Antibody response, Deep-seated infection, Immunoblot, ELISA

Date Deposited : 03 Aug 2011 10:42

Last Modified : 03 Aug 2011 10:42

Official URL: http://www.medsci.org/archive

Volume 2, Number 4, - 2005 , ISSN 1449-1907

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal