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.: Home > Animal Science Papers and Reports > 2009 > Volume 27 Number 1 > Amrit Kaur Bansal, Gurmail Singh Bilaspuri

Antioxidant effect of vitamin E on motility, viability and lipid peroxidation of cattle spermatozoa under oxidative stress

Amrit Kaur Bansal, Gurmail Singh Bilaspuri
Department of Animal Reproduction, Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India
Abstract :

Vitamin E is one of the major membrane protectants against reactive oxygen species ( ROS) and
lipid peroxidation (LPO). The study aimed at determining the optimum dose of vitamin E to  reverse free radical-mediated oxidative damage on motility, viability and LPO of bulls’sperm. Fresh semen of five local crossbred bulls was suspended in 2.9% sodium citrate, divided into equal fractions and subjected to vitamin E treatment (0, 1, 2, 2.5 mM) in the presence or absence of oxidative stress inducer, i.e ferrous ascorbate (FeAA, containing 150 ىM FeSO4 and 750 ىM ascorbic acid). All sperm suspensions were incubated at 37°C for 2 h. Treatment with FeAA reduced sperm motility and viability, but increased the LPO. All doses of vitamin E increased sperm motility and viability, but reduced LPO. However, 2 mM vitamin E was most effective. In conclusion, vitamin E reduced the LPO caused by FeAA, and improved sperm motility and viability in vitro under induced oxidative stress.

Keywords :
bulls / ferrous ascorbate / lipoperoxidation / oxidative stress / reactive oxygen species / sperm / vitamin E

Date Deposited : 04 Aug 2011 11:23

Last Modified : 04 Aug 2011 11:23

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Volume 27, Number 1, - 2009

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