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.: Home > Animal Science Papers and Reports > 2009 > Volume 27 Number 1 > W³adys³aw Kordan1, Agata Malinowska2,Marzena Mogielnicka1, Marek Lecewicz1

Application of mass spectrometry (MS) in the structural analysis of selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis (2-D PAGE)

W³adys³aw Kordan1, Agata Malinowska2,Marzena Mogielnicka1, Marek Lecewicz1
1 Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-917 0lsztyn-Kortowo, Poland 2 Polish Academy of Sciences Institute of Biochemistry and Biophysics, Pawiٌskiego 5a, 02-106 Warsaw, Poland
Abstract :

Two-dimensional electrophoresis (2-D PAGE) led to identification in the polypeptide maps of boar
seminal plasma of four conserved polypeptides with identical molecular weight of 24 kDA, and
different ranges of isoelectric point (pI): (1) 7.4-7.7, (2) 8.1-8.4, (3) 8.5-8.8 and (4) 9.2-9.4. In the
current study the molecular structures of these polypeptides were analysed, for the first time, by
mass spectrometry (LC – MS/MS). Computerized mass spectrometry analysis of the peptides
obtained after trypsin-digestion of the polypeptides demonstrated their similarity to the family
of spermadhesins (crystal structure of two members of the spermadhesin family), especially to
epididymal spermadhesin AWN-1. In addition, homology was found of peptides 3 and 4 with a
lysozyme C precursor (1,4-beta-N-acetylmuramidase C). The results presented might indicate the
participation of the analysed polypeptides in the processes accompanying fertilization.

Keywords :
boar / 2-D PAGE / mass spectrometry / proteins seminal plasma / spermadhesins

Date Deposited : 04 Aug 2011 11:24

Last Modified : 04 Aug 2011 11:24

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Volume 27, Number 1, - 2009

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