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.: Home > Animal Science Papers and Reports > 2009 > Volume 27 Number 2 > Tomasz Niemiec1*, Ewa Sawosz1, Marta Grodzik1, André Chwalibog2

High L-ascorbic acid content of diet modulates the non-specific immunity in rats

Tomasz Niemiec1*, Ewa Sawosz1, Marta Grodzik1, André Chwalibog2
Department of Animal Nutrition and Feed Science, Warsaw Agricultural University Ciszewskiego 8, 02-786 Warszawa, Poland 2 Department of Basic Animal and Veterinary Sciences, University of Copenhagen, Groennegaardsvej 3, 1870 Frederiksberg C, Denmark
Abstract :

This study was designed to examine the effect of high dose of L-ascorbic acid (L-AA) as a dietary
supplement for rats on the phagocytic activity and oxidative burst of non-specific immune cells. Thirty Wistar rats (225-245 g initial body weight) were divided into three groups fed diet supplemented with 0.0, 0.3, and 0.6% L-AA (0, 187 and 375 mg L-AA/kg feed) for 41 days. At the end of the experiment, blood samples were analysed for selected indicators of non-specific immunity. The application of 0.6% of LAA, as compared to the remaining two groups reduced phagocytic activity measured as a number of opsonized E. coli cells consumed by neutrophiles and monocytes. However, 0.6% of dietary AA supplementation led to increased phagocytic monocytes and neutrophiles percentage, which produced reactive oxygen species after stimulation with opsonized bacteria (E. coli), phorbol 12-myristate 13-acetate (PMA) and the chemotactic peptide N-formyl-Met-Leu-Phe (fMLP). These results indicate that megadose (0.6%) of dietary AA can reduce an oxygen-independent phagocytosis, but increase the number of neutrophiles and monocytes representing the oxygendependent mechanism of killing.

Keywords :
ascorbic acid / oxidative burst / phagocytic activity / rats / vitamin C

Date Deposited : 04 Aug 2011 13:24

Last Modified : 04 Aug 2011 13:24

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Volume 27, Number 2, - 2009

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