UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2008 > Volume 26 Number 3 > Marek Babicz1,**, Mariusz Pierzcha³a2, Pawe³ Urbaٌski2, Iwona Rozempolska-Ruciٌska3

An insertion/deletion polymorphism in the 3’UTR encoding region of the porcine prolactin (PRL) gene

Marek Babicz1,**, Mariusz Pierzcha³a2, Pawe³ Urbaٌski2, Iwona Rozempolska-Ruciٌska3
1 Department of Pig Breeding and Production Technology, University of Life Sciences of Lublin, Akademicka 13, 20-950 Lublin, Poland 2 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 3 Department of Biological Basis for Animal Production, University of Life Sciences of Lublin, Akademicka 13, 20-950 Lublin, Poland
Abstract :

A novel mutation, insertion/deletion (InDel) was found in the porcine prolactin gene exon 5 coding
for the 3پfUTR. Polymorphism was detected and identified using PCR-MSSCP and DNA  sequencing techniques, and then analysed with PCR-RFLP using HpyCH4III endonuclease. Inserted/deleted was an 11-bp TGTTTCTTAAC motive that occurred once or twice at position 201-222 in the amplified DNA fragment. The investigations covered 150 gilts of the Pu.awska breed from the herds under the Preserve Breeding Project. The frequencies of the inserted and deleted PRL gene variants were 0.34 and 0.66, respectively. The estimated value of heterozygosity (.) was 0.253 and the polymorphism information content (PIC) amounted to 0.348.

Keywords :
prolactin gene / polymorphisms / pigs / Pu³awska breed

Date Deposited : 08 Aug 2011 10:29

Last Modified : 08 Aug 2011 10:29

Official URL: http://www.ighz.edu.pl/

Volume 26, Number 3, - 2008

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal