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.: Home > Animal Science Papers and Reports > 2008 > Volume 26 Number 3 > Andrzej Dybus1,**, Ming-Huang Chang2, Yeong-Hsiang Cheng3, Iwona Szatkowska1

DNA polymorphism of the لA-globin gene in domestic pigeon

Andrzej Dybus1,**, Ming-Huang Chang2, Yeong-Hsiang Cheng3, Iwona Szatkowska1
1 Laboratory of Molecular Cytogenetics, Faculty of Biotechnology and Animal Science, Agricultural University of Szczecin, 71-460 Szczecin, Poland 2 Department of Veterinary Medicine, National Chia-Yi University, Chia-Yi, Taiwan 3 Institute of Biotechnology, Department of Animal Science, National I-Lan University, Taiwan
Abstract :

The study aimed at identifying the polymorphism of domestic pigeon لA-globin gene which can
be a potential homing marker in selection of racing pigeons. A total of 329 domestic pigeons were
genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) method. The PCR products were digested with 17 restriction endonucleases. One RFLP
– detected with HphI – was found in intron 1 and represented a C/T mutation. The second RFLP
– detected with BseLI – was a point mutation in the 3’UTR region of the gene (C/T mutation). The
polymorphism in the 3’UTR region of the pigeon لA-globin gene can potentially affect the stability
of mRNA and modify the gene expression. The mechanisms of haemoglobin function reflecting
variants of the لA-globin gene remain unknown.

Keywords :
domestic pigeons / genetic markers / globin gene / PCR-RFLP

Date Deposited : 08 Aug 2011 10:32

Last Modified : 08 Aug 2011 10:32

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Volume 26, Number 3, - 2008

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