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.: Home > Animal Science Papers and Reports > 2008 > Volume 26 Number 4 > Alma D. Alarcَn-Rojo1**, Isain Zapata1, Francisco J. Solيs2, Ivahan Lara1, Eduviges Burrola1, Lucina Cَrdoba2, Héctor Janacua-Vidales3

Microstructure of porcine meat with the Rendement Napole gene

Alma D. Alarcَn-Rojo1**, Isain Zapata1, Francisco J. Solيs2, Ivahan Lara1, Eduviges Burrola1, Lucina Cَrdoba2, Héctor Janacua-Vidales3
1 Faculty of Animal Science, Universidad Autَnoma de Chihuahua, Periférico Fco. R. Almada km 1, Chihuahua, Chih. 31031, México 2 Faculty of Medicine, Universidad Autَnoma de Chihuahua, Av. Colَn 1003 Chihuahua, Chih. 31350, México 3 Dept. of Veterinary Sciences, Biomedical Sciences Institute, Universidad Autَnoma de Cd. Juلrez. Henri Dunant 4016, Cd. Juلrez, Chih. 32310, México
Abstract :

The Rendement Napole gene (RN) consists of a mutation at the م3 subunit of AMP-activated protein kinase (AMPK) in pigs. AMPK works as an energy level sensor and controls numerous cellular pathways. The study aimed at identifying the differences in microstructure occurring between the normal porcine meat and porcine meat encoded by the RN gene. Using molecular techniques 194 commercial pigs were sampled to determine their genotypes. Meat from the semimembranosus muscle was examined using optic (OM) and transmission electron microscopy (TEM). The genotypes rnI/rnI, rnV/rnV, rnV/rnI, RN/rnV and RN/rnI were identified. OM revealed broadening of the sarcoplasmic space in RN animals, while TEM showed disordering of muscle fibres and alteration of the sarcomeric structure. Mitochondrial degradation was found in both RN and normal animals, however, the morphological changes observed seemed to be caused by the mutated AMPK.

Keywords :
AMPK / microstructure / muscle / pork / PRKAG3 / RN gene

Date Deposited : 08 Aug 2011 11:03

Last Modified : 08 Aug 2011 11:03

Official URL: http://www.ighz.edu.pl/

Volume 26, Number 4, - 2008

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