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.: Home > Animal Science Papers and Reports > 2007 > Volume 25 Number 2 > Gra؟yna Œwiderska-Ko³acz1, Jolanta Klusek1, Adam Ko³¹taj2

The effect of exogenous GSH, GSSG and GST-E on glutathione concentration and activity of selected glutathione enzymes in the liver, kidney and muscle of mice

Gra؟yna Œwiderska-Ko³acz1, Jolanta Klusek1, Adam Ko³¹taj2
1 Department of Animal Physiology, Institute of Biology, Œwiêtokrzyska Academy, Œwiêtokrzyska 15, 25-406 Kielce, Poland 2 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland
Abstract :

The experiment was conducted on randomly chosen 6 weeks-old mice, divided into four groups of 10 animals. The animals of control group were intraperitoneally injected with 250 ىl of 0.9% NaCl whereas those of three experimental groups with reduced glutathione (GSH), oxidized glutathione (GSSG) and glutathione ethyl ester (GST-E) at doses 250 ىg/kg body weight. Glutathione concentration as well as activity of glutathione enzymes was determined in liver, kidney and muscle tissues of control and experimental mice. Injections of GSH led to significant increase in the concentration of reduced glutathione in the liver only, increased activity of glutathione transferase and glutathione peroxidase in the liver, kidney and muscle, and of glutathione reductase in liver and kidney. Injections of GSSG caused the significant decrease in GSH concentration of the kidney while the activity of glutathione transferase rised in all three organs. The glutathione peroxidase rised only in the muscle. Injections of GST-E increased the glutathione concentration in the liver and muscle, and glutathione transferase activity in all three tissues. Simultaneously, the activity of glutathione peroxidase in both liver and kidney as well as of glutathione reductase in the muscle dropped.

Keywords :
enzyme / glutathione / liver / kidney / mice / muscle

Date Deposited : 08 Aug 2011 12:20

Last Modified : 08 Aug 2011 12:20

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Volume 25, Number 2, - 2007

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