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.: Home > Animal Science Papers and Reports > 2007 > Volume 25 Number 2 > Bo؟ena Witek1, Ewa Ochwanowska1, Danuta Baranowska1, Adam Ko³¹taj2

The effect of selection for body weight on the activity of lysosomal enzymes in the liver and kidney of mice

Bo؟ena Witek1, Ewa Ochwanowska1, Danuta Baranowska1, Adam Ko³¹taj2
1 Department of Animal Physiology, Institute of Biology, Œwiêtokrzyska Academy, Œwiêtokrzyska 15, 25-406 Kielce, Poland 2 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland
Abstract :

The study was conducted on 20 Swiss line male mice divided into two groups – control (n=10)
comprised of unselected animals (group C), and experimental (n=10) comprised of mice selected
over 24 generations for high live body weight gain (group S). Beginning from day 42 of life mice
of both groups received over a period of 14 days a standard pelleted feed containing 16% protein.
After 14 days the animals were killed, a lysosomal fraction of liver and kidney cells was obtained and analysed for the activity of acid phosphatase, lysosomal esterase, lysosomal lipase, â-glucuronidase, â-galactosidase, â-glucosidase, â-N-acetylo-hexosaminidase, leucine aminopeptidase, alanine aminopeptidase and cathepsins D and L. In S mice the activities of a majority of the lysosomal hydrolases examined were found to be higher than in C animals.

Keywords :
adaptation / mice / lysosomal enzymes / selection

Date Deposited : 08 Aug 2011 12:21

Last Modified : 08 Aug 2011 12:21

Official URL: http://www.ighz.edu.pl/

Volume 25, Number 2, - 2007

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