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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2007 > Volume 25 Number 4 > Daniel Lipiٌski1,2, Anna Maria Duszewska3,**, Joanna Zeyland1, Ewa Ma³y2, Witold Gawron3, Anna Rynkowska3, Zygmunt Reklewski3, Ryszard S³omski1,2

Obtaining transgenic bovine skin fibroblasts containing human interferon alpha gene

Daniel Lipiٌski1,2, Anna Maria Duszewska3,**, Joanna Zeyland1, Ewa Ma³y2, Witold Gawron3, Anna Rynkowska3, Zygmunt Reklewski3, Ryszard S³omski1,2
1 Department of Biochemistry and Biotechnology, Agricultural University of Poznaٌ, Wo³yٌska 35, 60-637 Poznaٌ, Poland 2 Polish Academy of Sciences Institute of Human Genetics, Strzeszyٌska 32, 60-479 Poznaٌ, Poland 3 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska; Poland
Abstract :

The study aimed at obtaining the heifer’s transgenic cell lines with integrated human interferon
alpha gene (IFNل) having in view cloning of Polish Red cattle. The gene construct used (pbLGIFNGFPBsd) contained the entire human interferon alpha gene under the bovine â-lactoglobulin promoter (bLG) and the green fluorescent protein gene (gfp) fused to the blasticidin resistance gene (bsd) under the human cytomegalovirus (CMV) immediate-early promoter. The gene construct was introduced by lipofection into heifer’s ear fibroblasts. The GFP- and Bsd-positive cell lines have been obtained. The molecular analysis (PCR) and cytogenetic characterization (FISH) confirmed obtaining the stable heifer’s transgenic cell lines with integrated human IFNل gene.

Keywords :
cattle / fibroblasts / interferon / lipofection / skin / transgenesis

Date Deposited : 08 Aug 2011 12:48

Last Modified : 08 Aug 2011 12:48

Official URL: http://www.ighz.edu.pl/

Volume 25, Number 4, - 2007

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