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.: Home > Animal Science Papers and Reports > 2006 > Volume 24 Number 1 > Stanis³aw Jَzef Rosochacki 1, 2, Antoni Baranowski1, Jَzef Klewiec1, Edyta Juszczuk-Kubiak1, Jaros³aw Po³oszynowicz1, Krystyna Wiciٌska1

A note on the protein metabolism in lambs fed the diet supplemented with bioplex and linseed or linseed oil

Stanis³aw Jَzef Rosochacki 1, 2, Antoni Baranowski1, Jَzef Klewiec1, Edyta Juszczuk-Kubiak1, Jaros³aw Po³oszynowicz1, Krystyna Wiciٌska1
1 Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 2 Chair of Sanitary Biology and Biotechnology, Bia³ystok Technical University, Wiejska 45a, 15-351 Bia³ystok, Poland
Abstract :

In trial R two groups of rams, beginning with 16 kg live weight (control group and experimental
group - Rc and Re, n=8 and n=9) were fattened with the concentrate containing 231 g CP and
12.0 MJ ME per kg dry matter. During 3 months Re lambs were given 3 g linseed with 3 g bioplex mineral supplement per day/ram. Both Rc and Re lambs were slaughtered at the age of 140 days. In trial E two groups of ewes, beginning with 8 kg live weight (control group and experimental group - Ec and Ee, n=10 and n=11) were fattened with the concentrate (207g CP and 12.5 MJ ME/kg DM). Moreover, Ee lambs were given 3 g linseed oil with 3 g bioplex mineral supplement per day/ewe during 5 months. Both Ec and Ee lambs were slaughtered at the age of 158 days. In longissimus dorsi muscle (LD) and in liver (L) measured were activities of cathepsin D (CatD), pepstatin-sensitive cathepsin D (PSCatD), acid autolytic activity (AAA), pepstatin-insensitive AAA (PIA) and leupeptin insensitive AAA (LIA). Moreover, determined were protein, RNA and DNA content in both tissues. No differences in tissues composition were found between Rc and Re lambs. In LD the 103 RNA/protein ratio was by 35.4% (P.0.000) and RNA concentration by 15.7% higher (P.0.007) in Ee than in Ec ewes, but protein/103DNA ratio was by 37.0% lower in Ee (P.0.001). In the liver, RNA/protein ratio was higher by 25.0% (P.0.01), but DNA concentration by 49.5% higher in Ee than in Ec group (P.0.000). Protein/DNA ratio was higher by 77.9% (P.0.000), but RNA/DNA higher by 42.2% inEc than in Ee ewes (P.0.000). Higher proteolytic activities occurred in rams: CatD (P.0.001), CatD+pepstatin (P.0.002) and LIA (P.0.000) in LD. CatD and PSCatD were higher in ewes LD (P.0.001 and P.0.003). In the liver, however, AAA (P.0.042) and LIA (P.0.016) were higher in rams while PIA in ewes (P.0.004). No differences were found between groups in both trials in inhibition of cathepsin D activity in LD and in liver at pH 3.75 judging by pepstatin inhibition and in AAA inhibition by pepstatin in LD and in the liver. In trial R, leupeptin inhibited by 23.3% more (P.0.01) in Rc than in Re rams, but in trial E no difference was found in inhibition rate between groups. The rise of the activity of mostly CatD (by 27.3%), PSCatD (by 12.3%) and LIA (by 69.5%) in the linseed fed rams (Re) and of CatD (by 18.0%) and PSCatD (by 23.9%) in the linseed oil fed ewes (Ee), both indicate a higher protein breakdown in their tissues, much more intensive by cathepsin D in rams than in ewes, where the higher thiol proteinases activity was found.

Keywords :
bioplex / cathepsin D / lambs / linseed / protein metabolism / thiol proteinases

Date Deposited : 08 Aug 2011 12:56

Last Modified : 08 Aug 2011 12:56

Official URL: http://www.ighz.edu.pl/

Volume 24, Number 1, - 2006

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