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.: Home > Animal Science Papers and Reports > 2006 > Volume 24 Number 2 > Grzegorz Grzybowski, Beata Prusak, Bartosz Romaniuk

A novel variant of the amelogenin gene (AMEL-X) in cattle and its implications for sex determination

Grzegorz Grzybowski, Beata Prusak, Bartosz Romaniuk
Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland
Abstract :

The amelogenin gene (AMEL) is often used in the research requiring sex determination of humans
and animals. In Bos taurus the most striking difference between the X- and Y-chromosomal AMEL
genes is the 63 bp deletion in exon 6 of the Y-linked copy. Size differences in the gene AMEL between the sex chromosomes are used for embryo sexing as well as in forensic casework, prenatal diagnosis, etc. The present paper shows that sex determination of cattle samples based on DNA sizing of gene AMEL is probably more complex than it was hitherto supposed. Used were 363 cows and 262 bulls of different breeds. According to a new polymorphism revealed in gene AMEL in chromosome X in both the homo- and heterogametic sex, the novel PCR fragments of atypical size can be detected.

Keywords :
cattle / amelogenin gene / chromosome X

Date Deposited : 08 Aug 2011 13:02

Last Modified : 08 Aug 2011 13:02

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Volume 24, Number 2, - 2006

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