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.: Home > Animal Science Papers and Reports > 2006 > Volume 24 Number 2 > Antoni Baranowski1, Stanis³aw Rosochacki2,4, Rafa³ Parada2, Kazimierz Jaszczak2, Janusz Zimny3, Jaros³aw Po³oszynowicz2

The effect of diet containing genetically modified triticale on growth and transgenic DNA fate in selected tissues of mice*

Antoni Baranowski1, Stanis³aw Rosochacki2,4, Rafa³ Parada2, Kazimierz Jaszczak2, Janusz Zimny3, Jaros³aw Po³oszynowicz2
1 Department of Animal Improvement, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 2 Department of Molecular Cytogenetics, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jatrzêbiec, 05-552 Wَlka Kosowska, Poland 3 Department of Plant Biotechnology and Cytogenetics, Plant Breeding and Acclimatization Institute, Radzikَw, 05-870 B³onie, Poland 4 Chair of Sanitary Biology and Biotechnology, Bia³ystok Technical University, Wiejska 45a, 15-351 Bia³ystok. Poland
Abstract :

C57Bl/6J mice were fed with pellets containing 20% of conventional >Bogo< triticale grain vs
pellets containing 20% transgenic triticale grain obtained from the same cultivar, but resistant to
BASTA herbicide with phosphonithricin as an active substance (control and experimental group,
respectively). Feeding regimen (with or without transgenic triticale) was maintained continuously in
both groups over five generations, and body weight of animals was recorded on day 21, 42, 63 and
91 of life. No significant inter-group differences in body weight were found. During the experiment,
mice of both groups showed no pathological manifestations, were found healthy and did not produce any visible skin changes. The PCR method used to analyse blood, kidneys, liver, spleen and thigh muscle of experimental animals did not show the presence of transgenic DNA (PCR product of 637bp) in any of the tissues. It is concluded that transgenic triticale used for experimental group had no effect on either growth of mice, or on the appearance of transgene in their tissues.

Keywords :
genetically modified organism / growth / mice / PCR /transgenic feed / triticale

Date Deposited : 08 Aug 2011 13:05

Last Modified : 08 Aug 2011 13:05

Official URL: http://www.ighz.edu.pl/

Volume 24, Number 2, - 2006

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