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.: Home > Animal Science Papers and Reports > 2005 > Volume 23 Number 1 > Cataldo Dario, Daria Carnicella, guido Bufano

note on the growth hormone (GH1-AluI) polymorphism in Podolian cattle in Southern Italy

Cataldo Dario, Daria Carnicella, guido Bufano
Department of Animal Health and Welfare, University of Study of Bari, Strada Provinciale per Casamassima km 3, 70010 Valenzano (Ba), Italy
Abstract :

Individual blood samples were collected from 132 unrelated Podolian cattle belonging to 13 farms
located in the inland territories of Southern Italy. Genomic DNA samples were genotyped for the
GH1-AluI polymorphism by Polymerase Chain Reaction . Restriction Fragment Length Polymorphism (PCR-RFLP). The mutation analysed causes the amino acid substitution leucineŁ¾¨valine at position 127 of the GH polypeptide. Allele frequencies for GH1 A and GH1 B were 0.85 and 0.15, respectively. Hardy-Weinberg equilibrium was found in the analysed animals. Due to the rather low frequency of GH1 B in the breed, a larger sample including a higher number of animals carrying GH1 B is necessary to assess possible association between GH1-AluI genotypes and beef traits in the Podolian cattle.

Keywords :
cattle / gene polymorphism / growth hormone / Podolian breed / PCR-RFLP

Date Deposited : 09 Aug 2011 10:49

Last Modified : 09 Aug 2011 10:49

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Volume 23, Number 1, - 2005

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