UMM Logo

Universitas Muhammadiyah Malang

Free Download Journals Directory

Nature and Science

.: Home > Nature and Science > 2010 > Volume 8 Number 3 > Sherein* I. Abd El-Moez 1 , Ahmed F.Y. 2 , Samy A.A. 1 , Aisha R.Ali 3

Probiotic Activity of L. acidophilus against Major Food-borne Pathogens Isolated from Broiler Carcasses.

Sherein* I. Abd El-Moez 1 , Ahmed F.Y. 2 , Samy A.A. 1 , Aisha R.Ali 3
. Department of Microbiology and Immunology - National Research Center Cairo Egypt. 2. Department of Animal Reproduction and A.I. - National Research Center Cairo Egypt. 3. Serology unit -Animal Health Institute Cairo Egypt *,,
Abstract :


C.jejuni, E.coli and S. typhimurium are the principal food borne pathogens in poultry  industry. The first 
experiment tested the effectiveness of different strains of Lactobacillus as  in vitro as probiotic against C. jejuni,  E.coli  O157 and S. typhimurium Result showed that  L.acidophilus isolated from colostrums of mare and goat  showed the widest inhibition zone against C. jejuni, E.coli O157 and S. typhimurium strains compared to the use of  L.acidophilus isolated from goat and cattle milk.  The second experiment evaluate the efficiency of   L.  acidophilus isolated from mare colostrums showing highest  in vitro inhibition activity against tested strains as   in  vivo probiotic against C. jejuni isolated from broiler carcasses. The result showed great inhibition of C. jejuni, E.coli  O157 and S. typhimurium strains by the use of  L.acidophilus in comparing to the use of antibiotics.    In the  second experiment; four groups of adult albino rats were used; group (1) control negative, group (2) rats orally  administrated by L. acidophilus only from the start of experiment till the 14th  day, group (3) rats challenged only 
with C. jejuni and group (4) orally administrated by L. acidophilus from the start of experiment till the 14th  day at the 7th  day they were challenged with  C. jejuni. Result showed that the third group showed the highest rate of  reisolation of  C.jejuni (0.80±0.16 from fecal swabs and 0.84±0.17 from the internal organs) as well as major  pathological lesions in the tested organs in the form of granulomatus reaction in the lung tissue infiltration of  inflammatory cells in the lung tissue thickening of wall of blood vessels with alveolar emphysema. Congestion  hemorrhages of renal blood capillaries and coagulative necrosis of the renal tissue as well as degeneration and  necrosis of hepatocytes with proliferation of fibrous tissue. The forth group pretreated with L. acidophilus Showed  lower rate of isolation of C.jejuni (0.08±0.02 from fecal swabs and 0.04±0.01 from internal organs. The pathological 
findings of the internal organs showed minor lesions in the form of interstitial pneumonia and inflammatory cellular 
infiltration in the lung Swelling and degeneration of renal epithelium and hepatocytic degeneration with infiltration 
of inflammatory cells. The second group which was only treated with  L.acidophilus showed no reisolation of 
C.jejuni as well as no pathological lesions were detected except a minor lesion in the liver in the form of diffused 
vacuolar degeneration in hepatocytes. Results develop a safe method for competing food borne pathogens in edible 
animals and suggest the need for probiotics to hinder the spread of highly pathogenic zoonotic bacteria transmitted 
by animal food by products. [Nature and Science 2010; 8(3):69-78]. (ISSN: 1545-0740).
Keywords :
C. jejuni; L. acidophilus; probiotics; in vivo, antibiotic sensitivity, rat.

Date Deposited : 12 Jan 2011 15:37

Last Modified : 12 Jan 2011 15:37

Official URL:

Volume 8, Number 3, March 2010 , ISSN 1545-0740

Full Text Original
Abstract : pdf doc