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.: Home > Animal Science Papers and Reports > 2005 > Volume 23 Number 1 > Pawe³ Brym, Stanis³aw Kamiٌski, El؟bieta Wَjcik

Polymorphism within the bovine prolactin receptor gene (PRLR)

Pawe³ Brym, Stanis³aw Kamiٌski, El؟bieta Wَjcik
Department of Animal Genetics, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
Abstract :

In marker-assisted selection (MAS) of dairy cattle certain genes are proposed as potential candidates associated with dairy performance traits. Among different candidates, prolactin receptor gene (PRLR) seems to be promising because of its crucial role in transmitting signal from lactogenic hormones to milk protein gene promoters. In this study nine PCR fragments representing most important functional domains of PRLR were screened for polymorphism. Using SSCP method one SNP (Aپ¨C) was found in intron 9. The SNP was deposited in GenBank AY484400 and AY339393 at position of 205 nt, for Jersey and Polish Black-and-White cattle, respectively. Allele frequency was estimated in 186 Polish Black-and-White (0.981 and 0.019 for A and C, respectively) and 138 Jersey (0.812 and 0.188 for A and C, respectively) cows. Preliminary analysis showed no significant ass ciations between PRLR genotypes and milk performance traits. However, Jersey cows of CC genotype produced more milk with higher protein content than those of AA and AC genotypes. Because of the low number of cows of CC genotype, it is necessary to investigate more numerous population of cattle in which all genotypes will be efficiently represented.

Keywords :
cattle / gene polymorphism / prolactin receptor gene / SSCP

Date Deposited : 09 Aug 2011 10:53

Last Modified : 09 Aug 2011 10:53

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Volume 23, Number 1, - 2005

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