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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2005 > Volume 23 Number 2 > Tadeusz Malewski1, Ma³gorzata Gajewska2, Lech Zwierzchowski1

Changes in DNA-binding activity of transcription factors in the differentiating bovine mammary gland

Tadeusz Malewski1, Ma³gorzata Gajewska2, Lech Zwierzchowski1
1 Department of Molecular Biology, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 2 Department of Physiology, Biochemistry, Pharmacology and Toxicology, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland
Abstract :

Our earlier studies carried out in silico demonstrated that different transcription factors have their
putative binding sites in the 5’-flanking regions of bovine milk protein genes. Now we extended our
study to include the experimental analysis of these transcription factors. This study on electrophoretic mobility shift assay (EMSA) of nuclear proteins derived from bovine mammary glands showed for the first time the presence there of CREB, NF-êB, Oct1 and Sp1 transcription factors. The pattern of DNA-protein complexes of Sp1 transcription factor significantly differed between virgin heifers and lactating cows. Transition from pregnancy to lactation was associated with changes in the DNAprotein binding patterns of NFI and Oct1 transcription factors.

Keywords :
cattle / casein genes / DNA-binding / transcription factor

Date Deposited : 09 Aug 2011 11:05

Last Modified : 09 Aug 2011 11:18

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Volume 23, Number 2, - 2005

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