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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2011 > Volume 23 Number 2 > Tadeusz Malewski1, Ma³gorzata Gajewska2, Lech Zwierzchowski1

Physiological state-dependent changes in transcription factor DNA-binding activities of the rabbit mammary gland

Tadeusz Malewski1, Ma³gorzata Gajewska2, Lech Zwierzchowski1
1 Department of Molecular Biology, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-552 Wَlka Kosowska, Poland 2 Department of Physiology, Biochemistry, Pharmacology and Toxicology, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland
Abstract :

Our earlier studies carried out in silico demonstrated that different transcription factors have their
putative binding sites in the 5’-flanking regions of rabbit milk protein genes. Now we extended this
study to include the experimental analysis of the transcription factors. This study of electrophoretic
mobility shift assay (EMSA) of nuclear proteins showed for the first time the presence of AP1, CREB, Myb, and Sp1 transcription factors in the rabbit mammary gland. The abundance of all transcription factors studied in the mammary gland changed during pregnancy-lactation-weaning cycle. The DNA-binding activity of the Myb transcription factor correlated well with the level of milk protein gene expression. The affinity of Myb to its binding sequence is methylation-dependent. One of CREspecific DNA-protein complexes was found only in nuclear protein from the lactating rabbits mammary gland. The amount of AP1 DNA-binding activity transiently decreased in the early involution. Transition from early to late involution was associated with the restoration of the AP1 activity, and with the decrease of the Myb and Sp1 protein-DNA binding activities.

Keywords :
casein genes / DNA-binding / rabbit / transcription factor

Date Deposited : 09 Aug 2011 11:15

Last Modified : 09 Aug 2011 11:21

Official URL:

Volume 23, Number 2, - 2011

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