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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2005 > Volume 23 Number 4 > Beata Prusak1, Tomasz Grzybowski2, Jaros³aw Bednarek2

Cytochrome b gene (cytb) in analysis of anonymous biological traces and its application in veterinary diagnostics and animal conservation

Beata Prusak1, Tomasz Grzybowski2, Jaros³aw Bednarek2
1 Department of Animal Immunogenetics, Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzêbiec, 05-522 Wَlka Kosowska, Poland 2 Department of Molecular and Forensic Genetics, The Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruٌ, M. Sk³odowskiej-Curie 9, 85-094 Bydgoszcz, Poland
Abstract :

Institutions and agencies which are to enforce veterinary and animal protection and conservation
regulations require means enabling identification of species origin of meat, trophies and other biological material. In this study a partial DNA sequence of cytochrome b gene (cytb) was used to identify three different anonymous samples: (1) the hair from the bird’s nest, (2) the blood stain found in the forest on snow cover and probably left by poachers, and (3) the fragment of the muscle from exotic reptile, provided by the Warsaw Zoological Garden. The sequences derived were compared with the sequences registered in GenBank, and species origin of samples was determined. The partial sequence of cytb adopted in the study proved to be useful for identification of animal species. Out of three samples subject to identification, one was found to belong to the goat, one to elk and another one to Python molurus, the latter two included in the list of protected species issued by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES).

Keywords :
animal conservation / cytochrome b / species identification

Date Deposited : 09 Aug 2011 12:31

Last Modified : 09 Aug 2011 12:31

Official URL: http://www.ighz.edu.pl/

Volume 23, Number 4, - 2005

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