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Animal Science Papers and Reports

.: Home > Animal Science Papers and Reports > 2003 > Volume 21 Number 1 > Andrzej W. Lipkowski1,2, Wies³awa Walisiewicz-Niedbalska1, Bo؟ena Patkowska-Soko³a3, Adam Opolski4, Robert Bodkowski3, Joanna Wietrzyk4, Marzena Pe³czyٌska4, Anna Nasulewicz4, Hanna Gwardiak1, Jacek Kwiatkowski1

In vitro anti-cancer properties of natural vs synthetic conjugated linoleic acid

Andrzej W. Lipkowski1,2, Wies³awa Walisiewicz-Niedbalska1, Bo؟ena Patkowska-Soko³a3, Adam Opolski4, Robert Bodkowski3, Joanna Wietrzyk4, Marzena Pe³czyٌska4, Anna Nasulewicz4, Hanna Gwardiak1, Jacek Kwiatkowski1
1 Industrial Chemistry Research Institute, Rydygiera 8, 01-793 Warszawa, Poland 2 Polish Academy of Sciences Medical Research Center, Pawiٌskiego 5, 02-106 Warszawa, Poland 3 Department of Sheep Breeding, Agricultural University of Wroc³aw, Ko؟uchowska 7, 51-631 Wroc³aw, Poland 4 Polish Academy of Sciences Institute of Immunology and Experimental Therapy, Rudolfa Weigla 12, 53-114 Wroc³aw, Poland
Abstract :

Anti-proliferative activity was investigated of C18:2 9cis,11trans (conjugated linoleic acid – CLA) isomer isolated from sheep milk fat. The research was conducted on sheep milk fatty acids in natural composition (FA-1) containing 2.0% of the C18:2 9cis,11trans CLA isomer and preparation enriched with CLA, containing up to 9.2% of the CLA isomer (FA-2). The process of enrichment of FA-1 was carried out in two steps. Step I was to remove mid-chain-length saturated fatty acids, primarily C16, by forming adducts with urea (to obtain FA-U), while in step II the short-chain acids were removed by way of supercritical extraction with CO2. The final preparation obtained (FA-2) contained 9.2% of the C18:2 9cis,11trans isomer. A natural sheep milk fatty acid composition (FA-1), the CLA-enriched product (FA-2) and a commercial CLA preparation (CP) were tested for anti-proliferative activity against the cells of human cervical carcinoma (KB), and leukaemia (HL-60). The ID50 dose (resulting in a 50% inhibition of
cell proliferation) of FA-2 was found lower than that of the CP preparation. The SRB test showedthe proliferation of the KB cells to have been inhibited to a greater degree by FA-2 than by gravimetrically identical (100 ىg/ml) doses of CP (85.3% vs 69.5%, respectively). Similarly, the MTT test showed the proliferation of the HL-60 cells to have been inhibited to a greater degree by FA-2 than by CP (99.0% vs 69.0%, respectively). Unmodified sheep milk fatty acids (FA-1) showed only a slight activity against the cells of both cancer lines (inhibition by about 10%).

Keywords :
carcinoma / conjugated linoleic acid (CLA) / leukaemia / sheep milk fat / supercritical extraction / urea

Date Deposited : 11 Aug 2011 13:38

Last Modified : 11 Aug 2011 13:38

Official URL: http://www.ighz.edu.pl/

Volume 21, Number 1, - 2003

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