UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2011 > Volume 7 Number 7 > Dario LirussiĆ’x and Mariana Matrajt

RNA Granules Present Only in Extracellular Toxoplasma Gondii Increase Parasite Viability

Dario LirussiĆ’x and Mariana Matrajt
Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
Abstract :

Toxoplasma gondii is an obligate intracellular parasite. When searching for a new cell to invade, the parasites have to confront the stress of being exposed to the extracellular en-vironment. The mechanisms by which T. gondii survives outside the host cells are poorly understood. In this work we show that extracellular parasites form mRNA aggregates with characteristics of stress granules. Intracellular tachyzoites or bradyzoites do not form mRNA granules. We tested different stimuli that trigger granule formation in vitro and discovered that a buffer that mimics the host cell cytosol ionic composition (high potassium) strongly induces granule formation, suggesting that the granules arise when the parasites come in contact with the host cell cytosol during egress. We examined the importance of granule formation for parasite viability and show that the parasite popu-lations that are able to form granules have a growth advantage, increased invasion, and decreased apoptosis in the extracellular environment. Overall, granule formation im-proves the fitness of extracellular parasites and increases the efficiency of the lytic cycle.

Keywords :
RNA FISH, T gondii, stress granules, extracellularity, parasite fitness.

Date Deposited : 24 Sep 2011 10:02

Last Modified : 24 Sep 2011 10:03

Official URL: http://www.biolsci.org/

Volume 7, Number 7, - 2011

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal