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International Journal Of Bilogical Sciences

.: Home > International Journal Of Bilogical Sciences > 2011 > Volume 7 Number 7 > Ping Zhou1, Shanli Zhai1, Xiang Zhou1, Ping Lin1, Tengfei Jiang1, Xueying Hu2, Yunbo Jiang3, Bin Wu3, Qingde Zhang1, Xuewen Xu1, Jin-ping Li4, Bang Liu1

Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo

Ping Zhou1, Shanli Zhai1, Xiang Zhou1, Ping Lin1, Tengfei Jiang1, Xueying Hu2, Yunbo Jiang3, Bin Wu3, Qingde Zhang1, Xuewen Xu1, Jin-ping Li4, Bang Liu1
1. Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 2. Pathology Lab of Animal, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 3. State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China; 4. Department of Medical Biochemistry and Microbiology, Biomedical Center, Uppsala University, Box 582, SE-751 23 Uppsala, Sweden.
Abstract :

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mainly the porcine alveolar macrophages (PAMs) and causes porcine reproductive and respiratory syn-drome (PRRS). Previous studies have analyzed the global gene expression profiles of lung tissue in vivo and PAMs in vitro following infection with PRRSV, however, tran-scriptome-wide understanding of the interaction between highly pathogenic PRRSV (HP-PRRSV) and PAMs in vivo has not yet been established. In this study, we employed Affymetrix microarrays to investigate the gene expression patterns of PAMs isolated from Tongcheng piglets (a Chinese indigenous breed) after infection with HP-PRRSV. During the infection, Tongcheng piglets exhibited typical clinical signs, e.g. fever, asth-ma, coughing, anorexia, lethargy and convulsion, but displayed mild regional lung damage at 5 and 7 dpi. Microarray analysis revealed that HP-PRRSV infection has af-fected PAMs in expression of the important genes involved in cytoskeleton and exocyto-sis organization, protein degradation and folding, intracellular calcium and zinc homeo-stasis. Several potential antiviral strategies might be employed in PAMs, including up-regulating IFN-induced genes and increasing intracellular zinc ion concentration. And inhibition of the complement system likely attenuated the lung damage during HP-PRRSV infection. Transcriptomic analysis of PAMs in vivo could lead to a better un-derstanding of the HP-PRRSV-host interaction, and to the identification of novel antiviral therapies and genetic components of swine tolerance/susceptibility to HP-PRRS.

Keywords :
microarray, HP-PRRSV-host interaction, infection, pulmonary alveolar macrophage, antiviral strategy

Date Deposited : 24 Sep 2011 10:04

Last Modified : 24 Sep 2011 10:04

Official URL: http://www.biolsci.org/

Volume 7, Number 7, - 2011

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