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Journal of Cancer

.: Home > Journal of Cancer > 2012 > Volume 3 Number 36 > Chemical Sciences Labo r- atory Building, 102 Varsity Way, Florida State University, Tallahassee, Florida 32306 - 4390, U.S.A

Identification of Potential Glycoprotein Biomarkers in Estrogen Receptor Positive (ER+) and Negative (ER - ) Human Breast Cancer Tissues by LC - LTQ/FT - ICR Mass Spectrometry

Chemical Sciences Labo r- atory Building, 102 Varsity Way, Florida State University, Tallahassee, Florida 32306 - 4390, U.S.A
Tel: +1-850-644-8683. Fax:+1-850-644-8281. Email: qxsang@chem.fsu.edu. Room 3501
Abstract :

Breast cancer is the second most fatal cancer in American women. To increase the life expectancy of patients with breast cancer new diagnostic and prognostic biomarkers and drug targets must be identified. A change in the glycosylation on a glycoprotein often causes a change in the function of that glycoprotein; such a phenomenon is correlated with cancerous transformation. Thus, glycoproteins in human breast cancer estrogen receptor positive (ER+) tissues and those in the more advanced stage of breast cancer, estrogen receptor negative (ER-) tissues, were compared. Glycoproteins showing differences in glycosylation were examined by 2-dimensional gel electrophoresis with double staining (glyco- and total protein staining) and identified by reversed-phase nano-liquid chromatography coupled with a hybrid linear quadrupole ion trap/ Fourier transform ion cyclotron resonance mass spectrometer. Among the identified glycosylated proteins are alpha 1 acid glycoprotein, alpha-1-antitrypsin, calmodulin, and superoxide dismutase mitochondrial precursor that were further verified by Western blotting for both ER+ and ER- human breast tissues. Results show the presence of a possible glycosylation difference in alpha-1-antitrypsin, a potential tumor-derived biomarker for breast cancer progression, which was expressed highest in the ER- samples.

Keywords :
Two - dimensional gel elec trophoresis; Estrogen receptor positive and negative breast cancer; Biomarkers; Alpha - 1 - antitrypsin; Fourier transform ion cyclotron resonance mass spe c- trometry; Proteomics

Date Deposited : 05 Jan 2015 14:12

Last Modified : 16 Jan 2015 10:54

Official URL: http://www.jcancer.org/

Volume 3, Number 36, - 2012

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