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Animal Bioresource in Japan

.: Home > Animal Bioresource in Japan > 2010 > Volume 59 Number 4 > Keiko KASHIWAGI1), Akihiko KASHIWAGI1), Atsushi KURABAYASHI2), Hideki HANADA1), Keisuke NAKAJIMA1), Morihiro OKADA1), Minoru TAKASE1) and Yoshio YAOITA1

Xenopus tropicalis: An Ideal Experimental Animal in Amphibia

Keiko KASHIWAGI1), Akihiko KASHIWAGI1), Atsushi KURABAYASHI2), Hideki HANADA1), Keisuke NAKAJIMA1), Morihiro OKADA1), Minoru TAKASE1) and Yoshio YAOITA1
1) Division of Embryology and Genetics, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University 2) Division of Diversification Mechanism, Institute for Amphibian Biology, Graduate School of Science, Hiroshima University
Abstract :

Studies using amphibians have contributed to the progress of life science including developmental biology and cell biology for more than one hundred years. Since the 1950s Xenopus laevis in particular has been used by scientists in many fields for experiments, resulting in the development of various techniques such as microsurgery on early embryos, biosynthesis of gene-encoded protein in oocytes by mRNA injection, misexpression experiments by mRNA injection into embryos, gene knockdown studies by injection of morpholino anti-sense oligonucleotide into fertilized eggs, transgenesis by the I-SceI meganuclease method, and so on. In this paper we will introduce Xenopus tropicalis as an alternative experimental animal. It has a shorter generation time and smaller diploid genome, together with whole-genome sequence data. The procedures available for Xenopus laevis can work well with Xenopus tropicalis, and embryos of both species develop at similar rates according to the developmental staging system of Nieuwkoop and Faber. Experimental systems of Xenopus tropicalis will pave the way for a new era of vertebrate genomics and genetics.

Keywords :
Xenopus genetic divergence, Xenopus laevis, Xenopus tropicalis (Silurana tropicalis) laboratory lines

Date Deposited : 16 Apr 2011 14:05

Last Modified : 16 Apr 2011 14:05

Official URL: http://www.jstage.jst.go.jp/article/expanim/59/4/59_395/_article

Volume 59, Number 4, April 2010 , ISSN 1881-7122

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