UMM Logo

Universitas Muhammadiyah Malang


Free Download Journals Directory


International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2013 > Volume 12 Number 3 > Janine Denadai, Antônio Carlos Paulillo, Elizabeth Moreira dos Santos Schmidt, Gislaine Regina Vieira Martins and Adriano de Oliveira Torres Carrasco

Investigation of the State of Virus Carrier of Newcastle Disease in Budgerigars (Melopsittacus undulatus)

Janine Denadai, Antônio Carlos Paulillo, Elizabeth Moreira dos Santos Schmidt, Gislaine Regina Vieira Martins and Adriano de Oliveira Torres Carrasco
Gislaine Regina Vieira Martins and Adriano de Oliveira Torres Carrasco 1,2 6 Alunos Programa de Pós-graduação em Medicina Veterinária da FCAV, Unesp, Jaboticabal, Brazil 1 Bolsistas FAPESP, Brazil 2 Departamento de Patologia Veterinária, FCAV, Unesp, Jaboticabal, Brazil 3 Research Fellow-CNPq-Brazil 4 Departamento de Clínica Veterinária, FMVZ, Unesp, Botucatu, Brazil 5 Universidade Estadual do Centro-Oeste, UNICENTRO, Paraná, Brazil
Abstract :

This study aimed to evaluate the importance of vaccination against Newcastle Disease (ND) in budgerigars (Melopsittacus undulatus) and to investigate the state of carrier of the virus (NDV) in this species. There were used 72 budgerigars distributed into four different experimental groups, vaccinated or not against ND: GI (Ulster 2C strain), GII (B1 strain), GIII (LaSota strain) and GIV (not vaccinated-control). At 11 months of age, all groups of birds were challenged with a pathogenic virus (NDV) suspension, EID50 = 108.15/0.1 mL. A group of Specific-Pathogen-Free (SPF) chickens were used as control of the virus. Cloacal swabs and blood samples were collected after 13 and 19 days post-challenge, respectively, for genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and antibody's level by the inhibition of hemagglutination test (HI). Budgerigars of all groups (including the control group-not vaccinated) did not demonstrate any signs of Newcastle Disease. They were refractory to the clinical disease with the NDV. Budgerigars from the control group (not vaccinated), showed antibody titers from HI test 13 and 19 days after challenge. Therefore, it was demonstrated the state of carrier of NDV in this species. The birds, from the vaccinated groups did not demonstrate genome viral excretion by RT-PCR and antibody titles were not detected by HI tests, respectively. It was also demonstrated the importance of the vaccination in the suppression of the state of carrier of NDV in budgerigars.

Keywords :
Budgerigars, Melopsittacus undulatus , vaccination, newcastle disease, NDV carrier

Date Deposited : 17 Mar 2015 11:06

Last Modified : 17 Mar 2015 11:06

Official URL: http://www.pjbs.org/ijps/back.htm

Volume 12, Number 3, - 2013 , ISSN 1682-8356

Download:
Full Text Original
Abstract : pdf doc
Home | Peta Situs | Admissions | Student Research | E-Journal
Universitas Muhammadiyah Malang © 2011
Developed by Infokom UMM
Last Update : 13 December 2016 20:46Kumpulan file jurnal