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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2013 > Volume 12 Number 5 > H.Y. Zhang, W.J. Yang, Y.Z. Luo and J.L. Han

Genetic Polymorphisms in a 1.2 kb Long Fragment within Intron 2 of Chicken UBTD2 Gene

H.Y. Zhang, W.J. Yang, Y.Z. Luo and J.L. Han
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China 1 CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources, Institute of Animal Science, 2 Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China International Livestock Research Institute (ILRI), P.O. Box 30709, Nairobi, Kenya
Abstract :

The widely expressed chicken UBTD2 gene in different types of tissues from embryonic to adult developmental stages implies its important role in regulating protein ubiquitination and delivery of ubiquitinated substrates, however, there is no specific study on the genomic DNA structure and function of chicken UBTD2 gene except a few invalidated SNPs derived from ESTs. In this study, a 1.2 kb long fragment within intron 2 of chicken UBTD2 gene was amplified and directly sequenced from a flock of 11 White Leghorn chickens. A total 15 sequences were identified from these birds, of which seven were homozygous but the remaining four were heterozygous. These new sequences were analyzed together with the homologous region in Gallus gallus reference genome version 71.4 (Chromosome 13: 8104760 - 8105979 in Galgal4). There were 10 polymorphic sites including seven transitions and three transversions, which defined four haplotypes in these 16 sequences. The Gallus gallus reference sequence formed a specific and distinct haplotype while the White Leghorn chickens carried three haplotypes at very similar frequencies of four, five and six sequences each. These data were the first piece of evidence of genetic polymorphisms present in the chicken UBTD2 gene which is therefore warranted for further investigation on the functional diversity in its complete genomic DNA sequence of different genetic backgrounds.

Keywords :
chicken, UBTD2 gene, intron 2, polymorphism

Date Deposited : 18 Mar 2015 10:30

Last Modified : 18 Mar 2015 10:30

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Volume 12, Number 5, - 2013 , ISSN 1682-8356

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