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International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2014 > Volume 13 Number 4 > Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah

Genetic Relatedness among Helicobacter pullorum Isolates from Broiler Chickens

Soe Soe Wai, A.A. Saleha, Z. Zunita, L. Hassan, A. Jalila and A.H. Shah
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia 2 Faculty of Animal Husbandry and Veterinary Sciences, 3 Sindh Agriculture University, Tandojam, Hyderabad, Pakistan
Abstract :

Helicobacter pullorum, an enterohepatic helicobacter with the ability to colonize the intestine and liver of avian species, mice and humans, is increasingly recognized as a food borne zoonotic pathogen. The present study aimed to determine the genetic similarity among H. pullorum using pulsed field gel electrophoresis (PFGE) with SacII and SmaI restriction endonucleases (REs) digestion. The H. pullorum were isolated from broiler chickens in eight farms. Within-farm genetic profiling of isolates showed close relationship (>95%); however genetic diversity was observed between isolates from different farms. Digestion of genome with SacII yielded two clusters comprised 16 pulsotypes, 8-14 DNA bands with a molecular weight ranging from 40 to 400 kb whereas SmaI digested genome produced two clusters with 14 pulsotypes, 7 to 13 DNA bands with molecular weight ranging from 40 to 250 kb. The RE SacII showed a higher discriminatory power compared to SmaI. In conclusion, within-farm genetic similarity of isolates suggested the same source of clonal origin.

Keywords :
Helicobacter pullorum ; chickens; genetic relationship; PFGE

Date Deposited : 20 Mar 2015 15:24

Last Modified : 20 Mar 2015 15:24

Official URL: http://www.pjbs.org/ijps/ab2538.htm

Volume 13, Number 4, - 2014 , ISSN 1682-8356

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