UMM Logo

Universitas Muhammadiyah Malang

Free Download Journals Directory

International Journal of Poultry Science

.: Home > International Journal of Poultry Science > 2014 > Volume 13 Number 9 > Hussein, A. Hussein, M.M. Emara, M.A. Rohaim, K. Ganapathy and A.M. Arafa

Sequence Analysis of Infectious Bronchitis Virus IS/1494 like Strain Isolated from Broiler Chicken Co-Infected with Newcastle Disease Virus in Egypt During 2012

Hussein, A. Hussein, M.M. Emara, M.A. Rohaim, K. Ganapathy and A.M. Arafa
Department of Virology, Faculty of Veterinary Medicine, Cairo University, Giza-12211, Egypt 1 Institute of Infection and Global Health, School of Veterinary Science, University of Liverpool, 2 Leahurst Campus, Neston, South W irral, CH64 7TE, UK Animal Health Research Institute, Dokki, Egypt
Abstract :

In the present study, we characterized IBV field isolate from a vaccinated broiler flock suspected to be co infected Newcastle disease virus (NDV). The flock demonstrated characteristic clinical signs, post mortem lesions and was positive to IBV and NDV by RT-PCR. The (S1) spike glycoprotein gene of (IBV) isolate was partially amplified by RT-PCR, then directly sequenced. The virus was designated (IBV/chicken/Egypt/VRLCU154/2012). Blast analysis, multiple alignments and phylogenetic analysis revealed that IBV isolate was in close relation to the IBV isolate IS/1494/06 (EU780077.2) with high nucleotide homology (97%). The sequence was submitted to NCBI GenBank with the accession No.JX893950. The results of the present study indicate that IS/1494 like strains might have a similar origin based on the genetic relationship among variants and reference strains.

Keywords :
IBV, broiler chickens, variant strains, sequence, mixed infection

Date Deposited : 24 Mar 2015 12:13

Last Modified : 24 Mar 2015 12:13

Official URL:

Volume 13, Number 9, - 2014 , ISSN 1682-8356

Full Text Original
Abstract : pdf doc