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Polymorphism in exon 2 of INHBB gene and its relationship with litter size in Jining Grey goats

M.X. Chu
gene polymorphism / goat / Inhibin β B gene / PC r -SSCP / prolificacy
Abstract :
The inhibin β
gene was studied as a candidate gene for the prolificacy of Jining Grey
goats. According to the sequence of bovine
gene, two pairs of primers were designed to
detect single nucleotide polymorphism in exon 2 of
gene in high-prolificacy (Jining Grey)
and low-prolificacy (Inner Mongolia Cashmere and Angora) goats by polymerase chain reaction-
single strand conformation polymorphism method. Only the amplified products of primer P2 showed
polymorphism. Three genotypes (AA, AB and BB) were identified in Jining Grey and two (AB and
BB) in Inner Mongolia Cashmere and Angora goats. Sequencing revealed one single nucleotide
mutation (A→G) at base 782 of exon 2 of
gene in BB genotype compared to genotype AA.
In Jining Grey goats the frequencies of A and B alleles were 0.318 and 0.682 while of AA, AB and
BB genotypes reached 0.068, 0.500 and 0.432, respectively. The Jining Grey goats remained in
Hardy-Weinberg equilibrium (chi-square
= 3.36, P = 0.1861). These of genotype AA delivered by
0.73 (P<0.05) or 0.84 (P<0.05) kids more than those with genotype AB or BB, while does of genotype
AB  by 0.11 (P>0.05) kids more than did does of genotype BB
Keywords :
gene polymorphism / goat / Inhibin β B gene / PC r -SSCP / prolificacy

Date Deposited : 01 Apr 2015 15:18

Last Modified : 01 Apr 2015 15:18

Official URL:

Volume 30, Number 1, - 2012

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