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Frequency of BoLA-DRB3 alleles in Polish Holstein-Friesian cattle

olanta Oprządek
Polish Academy of Sciences Institute of Genetics and Animal Breeding, Jastrzębiec, 05-552 Magdalenka, Poland
Abstract :
The aim of the study was to describe polymorphism of BoLA-DRB3.2 in Polish Holstein-Friesians.
Alleles were identified according to PCR-RFLP procedure using a modification to the van Eijk’s
proposal [1992]. Restriction patterns were obtained with BstYI, HaeIII and RsaI enzymes. The
study presented variability of all the alleles estimated in two herds. 752 animals were genotyped
and informative according to previously described RFLP patterns. The most frequent homozygous
animals were DRB3.2*24. The frequency was 0.066 and 0.078 in the sampled herds. No DRB3.2*03
nor DRB3.2*28 homozygous animals were found in one herd as opposite to another herd where
homozygous animals DRB3.2*03 occurred with frequency 0.26 and animals DRB3.2*28 occurred
with frequency 0.59. Homozygous DRB3.2*gba was not observed in that herd. The differences in
allele frequencies may be associated with herd production and fitness status.
Keywords :
BoLA-DRB3 / Polish Holstein-Friesian / polymorphism / semi-nested PCR

Date Deposited : 01 Apr 2015 15:23

Last Modified : 01 Apr 2015 15:29

Official URL:

Volume 30, Number 2, - 2012

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